|
TOOLS FOR RBTI TESTING OF SALIVA AND
FRESHLY VOIDED URINE |
| FIRST: study mass of urine
with light to estimate cell debris...RECORD CELL DEBRIS |
| Put one drop of saliva in
well depressions D & E... |
| Put four drops of
ammonia nitrogen
reagent in well depression F... |
| Put six drops
Universal Extracting
Solution (UES) in test tube or nurse's pill dispenser cup... |
| Put one drop of urine in
well depression A & B; one drop of urine in test tube (cup) and two drops of
urine on refractometer prism... |
| Close cover plate on
refractometer
and read scale...RECORD BRIX |
| Agitate mixture in test tube (cup) for 1
minute or 120 squeezes... |
| Put one drop of agitated mixture
in well depressions C and F... |
| Wait 15 seconds and compare
well depression F to ammonia nitrogen card...RECORD AMMONIA
PPM |
| Drop four drops of
nitrate
nitrogen reagent into well depression C at 4 points of compass... |
| Put one drop of
Bromthymol Blue pH
reagent in well depressions A & D... |
| Observe the pH colors forming in
well depressions A
& D. If either is on the acid side (below 6.40, yellowish) add one
drop of Chlorphenol Red pH reagent to well depression next to it (B, E, or both
if needed)... |
| If pH colors in either A or D or
both are on the alkaline side (above 6.40---bluish) add one drop of
Phenol Red pH reagent to the well depression next to it (B, E or both if
necessary)... |
| NOTE: if after adding
Chlorphenol
Red pH reagent to B and/or E, and it appears that the urine or
saliva is very acid (less than 5.40), use locations G and/or H to
add either one drop of urine and/or saliva and then one drop of Bromcresol Green
pH reagent... |
| Read urine and saliva pH using
appropriate card matched to reagent...RECORD SALIVA pH AND URINE
pH |
| Use conductivity (EC) meter to obtain µsiemens
conductivity reading of urine and divide by 700...RECORD SALTS
NUMBER
|
Compare well depression C with
nitrate nitrogen card after 4-6 minutes...RECORD NITRATE NITROGEN
PPM
 |